Anti-HA Tag Mouse mAb
- 100 μL
产品信息
蛋白质全称 | HA标签 |
别名 | HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain |
免疫原 | KLH偶联的YPYDVPDYA合成肽 |
抗体亚型 | IgG1, κ |
克隆号 | 2A5H5 |
纯化方式 | 亲和纯化 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | Species independent | 1: 500-1: 1000 | HA标签融合蛋白 |
| IP 免疫沉淀 | Species independent | 1: 50-1: 200 | 转染HEK-293F细胞 |
背景
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.
图像
| WB检测HA Tag(货号 GB12939). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗小鼠IgG (H+L) (货号GB23301), 1: 5000稀释, 室温孵育1小时. |
| 免疫共沉淀转染的HEK-293F细胞裂解液，使用HA标签抗体（免疫沉淀抗体：GB12939，1:100；检测抗体：GB12939，1:3000） |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. |
注意:
1.本产品仅供研究使用.
2.建议用一抗稀释液(G2025)稀释本产品.
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