产品信息

蛋白质全称	Whirlin蛋白
别名	Whrn, Kiaa1526, CIP98, DFNB31, USH2D, WI
Uniprot ID	Q80VW5
免疫原	小鼠DFNB31重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	98 kDa / 120 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 1000-1: 2000	K562细胞, U266细胞, PC3细胞

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背景

Involved in hearing and vision as member of the USH2 complex . Necessary for elongation and maintenance of inner and outer hair cell stereocilia in the organ of Corti in the inner ear . Involved in the maintenance of the hair bundle ankle region, which connects stereocilia in cochlear hair cells of the inner ear . In retina photoreceptors, required for the maintenance of periciliary membrane complex that seems to play a role in regulating intracellular protein transport .

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图像

WB检测DFNB31蛋白(货号 GB112690). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.