产品信息

蛋白质全称	细胞色素b-c1复合体Rieske蛋白（线粒体）
别名	Complex III subunit 5, Cytochrome b-c1 complex subunit 5, Rieske iron-sulfur protein, RISP, Rieske protein UQCRFS1, Subunit 9, Complex III subunit IX, Uqcrfs1, UQCRFS1 MTS
Uniprot ID	Q9CR68
免疫原	小鼠UQCRFS1/RISP重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	29 kDa / 26 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 2000-1: 4000	心, 骨骼肌

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背景

UQCRFS1 is an iron-sulfur Rieske protein belongs to major subunits of cytochrome b6f complex of photosynthetic membranes and is encoded by petA gene. A hydrophobic stretch next to N-terminus of the protein is considered as a transmembrane anchor for the Rieske protein. It had been shown that the Rieske protein is involved in the quinol binding. In plants it is essential protein for photoautotrophism. Confers resistance to photo-oxidative damages by contributing to the thermal dissipation of light energy and to lumenal acidification (increase of pH gradient).

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图像

	WB检测UQCRFS1/RISP蛋白(货号 GB112567). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 2000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.
	WB检测UQCRFS1/RISP蛋白(货号 GB112567). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 2000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.