产品信息

蛋白质全称	三激酶/FMN环化酶
别名	Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing), FAD-AMP lyase ATP-dependent dihydroxyacetone kinase, Glycerone kinase, Triokinase, FAD-AMP lyase (cyclizing) , DHA kinase, DAK,FMN cyclase, NET45, Tkfc
Uniprot ID	Q8VC30
免疫原	KLH偶联的小鼠DAK合成肽
抗体亚型	IgG
预测分子量./观测分子量.	60 kDa / 60 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 500-1: 1000	肝, 肾, 睾丸

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背景

DAK is a member of the family of dihydroxyacetone kinases, which have a protein structure distinct from other kinases. The product of this gene phosphorylates dihydroxyacetone, and also catalyzes the formation of riboflavin 4',5'-phosphate (aka cyclin FMN) from FAD. Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. Represses IFIH1-mediated cellular antiviral response.

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图像

WB检测DAK蛋白(货号 GB111568). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.