产品信息

蛋白质全称	谷胱甘肽S-转移酶A1
别名	13-hydroperoxyoctadecadienoate peroxidase, Androst-5-ene-3, 17-dione isomerase, GST class-alpha member 1, Gsta1, Gsta, Gstya, Glutathione S-transferase Ya, Glutathione S-transferase Ya1, Glutathione S-transferase A1, N-terminally processed
Uniprot ID	P13745
免疫原	小鼠GSTA1重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	26 kDa / 26 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 500-1: 1000	肝, 肾

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背景

This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver. In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity thereby protecting the cells from reactive oxygen species and the products of peroxidation.

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图像

WB检测GSTA1蛋白(货号 GB111400). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.