产品信息

蛋白质全称	二甲基苯胺单加氧酶3
别名	Dimethylaniline oxidase 3, FMO form 2, FMO3, FMOII, TMAU, Hepatic flavin containing monooxygenase 3, Trimethylamine monooxygenase, Flavin containing monooxygenase 3
Uniprot ID	P97501
免疫原	KLH偶联的小鼠FMO3合成肽
抗体亚型	IgG
预测分子量./观测分子量.	61 kDa / 61 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠	1: 300-1: 500	肝, 肾

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背景

Microsomal flavin-containing monooxygenase (FMO) catalyzes the FAD-, NADPH-, and O2-dependent oxidation of a large number of xenobiotics containing soft nucleophiles,including alkaloids, pesticides, and pharmaceutical substances.Based on the cDNA sequence, the FMOs are classified into five subfamilies (FMO1 to FMO5).In human beings ,FMO3 is predominant in the adult liver,but not appear sex-dependent in the tissue.In the mouse liver,its expression has been shown to be sex-dependent and expressed only in females.Defects in FMO3 are the cause of trimethylaminuria (TMAU).This antibody is specific to FMO3.

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图像

WB检测FMO3蛋白(货号 GB113169). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.