Anti-TATA binding protein TBP antibody (HRP conjugated)
- 100 μL
产品信息
蛋白质全称 | TATA框结合蛋白 |
别名 | GTF2D, GTF2D1, SCA17, TATA-binding factor, TATA-box factor, TATA sequence binding protein, TBP, TF2D, TFIID |
Uniprot ID | P29037 |
免疫原 | KLH偶联的小鼠TBP合成肽 |
预测分子量./观测分子量. | 35 kDa / 35 kDa |
纯化方式 | 亲和纯化 |
偶联 | 辣根过氧化物酶 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 小鼠, 大鼠 | 1: 200-1: 500 | 睾丸 |
| 孵化条件 | 封闭后, 将膜与HRP标记的抗TATA binding protein TBP 抗体 (ZB113810-HRP)在室温（20-25℃）孵育3-4小时或4℃过夜, 随后用TBST缓冲液洗涤3次, 每次5分钟. | ||
背景
The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene.
图像
| WB检测TATA binding protein TBP蛋白(货号 ZB113810-HRP). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. 抗体: HRP标记的抗TATA binding protein TBP蛋白 抗体 1: 500稀释, 4℃ 孵育过夜. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.产品颜色为浅黄色. |
注意:
1.本产品仅供研究使用.
2.建议用一抗稀释液(G2025)稀释本产品.
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