Anti-Transferrin antibody (HRP conjugated)
- 50 μL
产品信息
蛋白质全称 | 血清转铁蛋白 |
别名 | Transferrin, Beta-1 metal-binding globulin, Liver regeneration-related protein LRRG03, Siderophilin, Tf |
免疫原 | KLH偶联的大鼠Transferrin合成肽 |
Uniprot ID | P02787, Q921I1, P12346 |
偶联 | 辣根过氧化物酶 |
纯化方式 | 亲和纯化 |
预测分子量. / 观测分子量. | 77 kDa / 77-80 kDa |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 小鼠 | 1: 500-1: 800 | 肝 |
| 孵化条件 | 封闭后, 将膜与HRP标记的抗Transferrin 抗体 (ZB111312-HRP)在室温（20-25℃）孵育3-4小时或4℃过夜, 随后用TBST缓冲液洗涤3次, 每次5分钟. | ||
背景
Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins.It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum.
图像
| WB检测Transferrin蛋白(货号 ZB111312-HRP). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. 抗体: HRP标记的抗Transferrin蛋白 抗体 1: 500稀释, 4℃ 孵育过夜. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.产品颜色为浅黄色. |
注意:
1.本产品仅供研究使用.
2.建议用一抗稀释液(G2025)稀释本产品.
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