产品信息

蛋白质全称	微管蛋白α-1A链
别名	TUBA1A, B-ALPHA-1, LIS3, TUBA3, tubulin alpha 1a,TUBA1B, K-ALPHA-1, tubulin alpha 1b
Uniprot ID	P68369
免疫原	小鼠alpha Tubulin重组蛋白
预测分子量./观测分子量.	50 kDa / 55 kDa
纯化方式	亲和纯化
偶联	辣根过氧化物酶

---

应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 3000-1: 6000	HeLa细胞, Hep G2细胞, 肺
孵化条件	封闭后, 将膜与HRP标记的抗alpha Tubulin 抗体(ZB15200-HRP)在室温（20-25℃）孵育3-4小时或4℃过夜, 随后用TBST缓冲液洗涤3次, 每次5分钟.

---

背景

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species.

---

图像

WB检测alpha Tubulin蛋白(货号 ZB15200-HRP). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. 抗体: HRP标记的抗alpha Tubulin蛋白 抗体 1: 3000稀释, 4℃ 孵育过夜.

---

储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.产品颜色为浅黄色.

---

注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.