产品信息

蛋白质全称	甘露糖-6-磷酸受体（非阳离子依赖型）
别名	CI Man-6-P receptor, CI-MPR, M6PR, 300 kDa mannose 6-phosphate receptor, MPR 300, nsulin-like growth factor 2 receptor, IGF-II receptor, CD222, Igf2r, M6P/IGF2R
Uniprot ID	Q07113
免疫原	KLH偶联的小鼠M6PR (cation independent) Uniprot ID Q07113合成肽
抗体亚型	IgG
预测分子量./观测分子量.	274 kDa / 300 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 500-1: 1000	肝, 肾, 心

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背景

IGF2R, also named as MPRI, CI Man-6-P receptor, CI-MPR, M6PR, IGF-II receptor, M6P/IGF2R, MPR 300 and CD222, belongs to the MRL1/IGF2R family. IGF2R transports of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. IGF2R is a lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.

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图像

WB检测M6PR (cation independent)蛋白(货号 GB111801). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.