产品信息

蛋白质全称	突触体相关蛋白25
别名	Snap25, SNAP25, CMS18, RIC-4, RIC4, SEC9, SNAP, SNAP-25, bA416N4.2, dJ1068F16.2, SUP, Synaptosome associated protein 25kDa, Synaptosome associated protein 25
Uniprot ID	P60879
免疫原	小鼠SNAP25重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	23 kDa / 25 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 1000-1: 2000	脑, 海马区, 大脑皮层

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背景

Synaptosomal associated protein 25 kDa (SNAP-25) is a widely distributed membrane-associated protein in the brain, mainly localized in nerve terminals. SNAP-25 is a component of the trans-SNARE complex, which is proposed to account for the specificity of membrane fusion and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP-25 regulates plasma membrane recycling through its interaction with CENPF.

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图像

WB检测SNAP25蛋白(货号 GB11678). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.