产品信息

蛋白质全称	GTP酶IMAP家族成员4
别名	Immunity-associated nucleotide 1 protein, IAN-1, Immunity-associated protein 4, Gimap4, Ian1, Imap4
Uniprot ID	Q99JY3
免疫原	KLH偶联的小鼠GIMAP4合成肽
抗体亚型	IgG
预测分子量./观测分子量.	38 kDa / 38 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠	1: 500-1: 1000	脾, 胸腺, 卵巢

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背景

GIMAP4, also named as hIAN1 or HIMAP4, is a member of GIMAP family. Among GIMAP family, GIMAP4 is the only member reported to have true GTPase activity, and its transcription is found to be differentially regulated during early human CD4+ T helper (Th) lymphocyte differentiation. GIMAP4 has been previously connected mainly with T- and B-cell development and survival and T-cell apoptosis.

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图像

WB检测GIMAP4蛋白(货号 GB111408). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.