产品信息

蛋白质全称	纤维蛋白原样2凝血酶原酶
别名	Cytotoxic T-lymphocyte-specific protein, Fibrinogen-like protein 2, Prothrombinase, Fgl2, Fiblp, pT49, T49, fibrinogen like 2
Uniprot ID	P12804
免疫原	小鼠FGL2/Prothrombinase重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	49 kDa / 64 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1000	Raji细胞, HepG2细胞, SW480细胞

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背景

FGL2 (fibrinogen like protein 2) is a 70 kDa glycoprotein that belongs to the fibrinogen-related superfamily of proteins which are involved in coagulation, cell adhesion, and transendothelial migration. FGL2 is an important immune regulator of both innate and adaptive response. It is present on the surface of macrophages and endothelial cells, and can be constitutively secreted by CD4+ CD8+ T cell. FGL2 forms a tetrameric complex which is stabilized by interchain disulfide bonds and it exerts immunosuppressive effects on both T cell proliferation and dendritic cell maturation.

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图像

WB检测FGL2/Prothrombinase蛋白(货号 GB111587). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.