产品信息

蛋白质全称	组织蛋白酶L2
别名	cathepsin L2, Cathepsin U, Cathepsin V, CATL2, CTSL2, CTSU, CTSV
Uniprot ID	O60911
免疫原	KLH偶联的人Cathepsin V合成肽
抗体亚型	IgG
预测分子量./观测分子量.	37 kDa / 37 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	大鼠	1: 300-1: 1000	肾, 睾丸

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背景

Cathepsin V is a lysosomal cysteine protease which has an important role in corneal physiology.Cathepsin V was found to be differentially expressed in relation to pigmentary skin backgrounds, relative to darkly pigmented skins. It is expressed in primary keratinocytes, melanocytes, and HaCaT keratinocytes, but not in primary fibroblasts. The purified cathepsin L2 was a high molecular weight proteinase of about 78 kDa, but it was unstable to SDS and reducing conditions, which dissociated parts of the proteinase into 66 kDa, 31 kDa and 26 kDa fragments and it can form a complex of 70 kDa with an unidentified subunit.

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图像

WB检测Cathepsin V蛋白(货号 GB113344). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.