产品信息

蛋白质全称	环指蛋白151
别名	ring finger protein 151, RNF151
Uniprot ID	Q9CQ29
免疫原	小鼠RNF151重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	27 kDa / 33 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1500	THP-1细胞

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背景

RNF151 also termed as ring finger protein 151 is a 245 amino acid protein, which contains one Ring-type zinc finger and one TRAF-type zinc finger. RNF151, with, a putative nuclear localization signal (NLS)was exclusively expressed in the mouse testis and developmentally regulated during spermatogenesis. While RNF151 mRNA was present in round spermatids, its protein was expressed in elongating spermatids of the stage VIII-IX seminiferous tubules.

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图像

WB检测RNF151蛋白(货号 GB114169). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.