产品信息

蛋白质全称	真核翻译起始因子3亚基L
别名	eIF3l, Eukaryotic translation initiation factor 3 subunit 6-interacting protein, EIF3L, EIF3EIP, HSPC021, HSPC025, MSTP005
Uniprot ID	Q8QZY1
免疫原	小鼠eIF3L重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	67 kDa / 67 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1000	HEK293细胞, HeLa细胞, A549细胞

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背景

The eIF3L is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eukaryotic initiation factor-3 (eIF3) scaffolding structure function to control the assembly of the 40S ribosomal subunit. Association of eIF3 proteins with the 40S ribosomal subunit stabilizes eIF2-GTP-Met-tRNAiMet complex association and mRNA binding, and promotes dissociation of 80S ribosomes into 40S and 60S subunits, thereby promoting the assembly of the pre-initiation complex.

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图像

WB检测eIF3L蛋白(货号 GB111534). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.