产品信息 蛋白质全称 核因子κB激酶抑制因子α亚基 别名 Chuk, CHUK, IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16, Conserved helix-loop-helix ubiquitous kinase, Component of inhibitor of nuclear factor kappa B kinase complex Uniprot ID Q60680 免疫原 小鼠IKKα重组蛋白 抗体亚型 IgG 预测分子量./观测分子量. 85 kDa / 85 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 人, 小鼠, 大鼠 1: 500-1: 1000 胸腺, 睾丸 背景 IKK-α is a member of the serine/threonine protein kinase family and forms a complex in the cell with IKK-βand NEMO. NF-κB transcription factors are normally held in an inactive state by the inhibitory proteins IκBs. IKK-α and IKK-β phosphorylate the IκB proteins, marking them for degradation via ubiquitination and allowing NF-κB transcription factors to go into the nucleus. Once activated, NF-κB transcription factors regulate genes that are implicated in many important cellular processes, including immune response, inflammation, cell death, and cell proliferation. 图像 WB检测IKK alpha蛋白(货号 GB11923). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. WB检测IKK alpha蛋白(货号 GB11923). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.