Product Information Description IKB alpha rabbit polyclonal Protein full name NF-kappa-B inhibitor alpha Synonyms NFKBIA, IKBA, MAD-3, NFKBI,NFKB inhibitor alpha Immunogen KLH conjugated Synthetic peptide corresponding to Rat IKB alpha. Isotype IgG Purity Affinity purification Predicted MW. 36 kDa Observed MW. 36 kDa Uniprot ID P25963 , Q9Z1E3 , Q63746 Applications applications species dilution Positive tissue WB Human, Mouse, Rat 1: 500-1: 1000 spleen, skin, lung Background Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting. Images Western blot analysis of IKB alpha (GB13212-1) at dilution of 1:1000. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: A431 cell lysate Lane 4: Mouse spleen tissue lysate Lane 5: Mouse skin tissue lysate Lane 6: Mouse lung tissue lysate Lane 7: Rat spleen tissue lysate Lane 8: Rat skin tissue lysate Lane 9: Rat lung tissue lysate Storage Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. NOTE: 1.This product is intended for research only. 2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （ G2025 ）.