Product Information Description ERK1/2 rabbit polyclonal Protein full name ERK1/2 Synonyms Mitogen-activated protein kinase 3, MAP kinase 3,MAPK 3,ERT2, Extracellular signal-regulated kinase 1, ERK-1,Insulin-stimulated MAP2 kinase,MAP kinase isoform p44, p44-MAPK,Microtubule-associated protein 2 kinase,p44-ERK1,MAPK3,ERK1,PRKM3 Immunogen Synthetic peptide, corresponding to amino acids 320-370 of Human ERK1 Isotype IgG Purity Affinity purification Subcellular location Nucleus Uniprot ID P27361, P28482 Applications Applications Species Dilution Positive Tissue IHC Human, Mouse, Rat 1: 50-1: 200 eye, hepatoma, cervix, lymph Background The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and Threonine sites mapping within a characteristic Thr- Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phos-phorylate downstream components on Serine and Threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6. Images Storage Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. NOTE: 1.This product is intended for research only. 2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （ G2025 ）.