产品信息 蛋白质全称 酪氨酸蛋白激酶Lyn 别名 LYN, LYN proto-oncogene, Src family tyrosine kinase, JTK8, p53Lyn, p56Lyn Uniprot ID P25911 免疫原 小鼠Tyrosine-protein kinase Lyn重组蛋白 抗体亚型 IgG 预测分子量./观测分子量. 59 kDa / 56 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 人, 小鼠, 大鼠 1: 500-1: 1000 肝, 脾, 肺 背景 Tyrosine-protein kinase Lyn is a member of the Src family of protein tyrosine kinases, which is mainly expressed in hematopoietic cells, in neural tissues, liver, and adipose tissue. In various hematopoietic cells, Lyn has emerged as a key enzyme involved in the regulation of cell activation. In these cells, a small amount of LYN is associated with cell surface receptor proteins, including the B cell antigen receptor (BCR), CD40 or CD19. The abbreviation Lyn is derived from Lck/Yes novel tyrosine kinase, Lck and Yes also being members of the Src kinase family. 图像 WB检测Lyn蛋白(货号 GB11646). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. WB检测Lyn蛋白(货号 GB11646). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.