产品信息 蛋白质全称 核糖体蛋白S6激酶α-5 别名 Rps6ka5, RPS6KA5, MSK1, MSPK1, RLPK, Ribosomal protein S6 kinase A5, Mitogen and stress activated protein kinase 1 Uniprot ID Q8C050 免疫原 小鼠MSK1重组蛋白 抗体亚型 IgG 预测分子量./观测分子量. 90 kDa / 90 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 人, 小鼠, 大鼠 1: 500-1: 1000 脑, 肺, 胸腺 背景 Ribosomal protein S6 kinase alpha-5 is an enzyme encoded by the RPS6KA5 gene. MSK1 is a mitogen and stress activated protein kinase 1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK1 can be activated by ERK1/2 and SAPK2/p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG14 in response to mitogen and stress. 图像 WB检测MSK1蛋白(货号 GB11857). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.