产品信息 蛋白质全称 KiSS-1受体 别名 KiSS-1R, G-protein coupled receptor 54, G-protein coupled receptor OT7T175, mOT7T175, Kisspeptins receptor, Metastin receptor, Kiss1r, Gpr54 Uniprot ID Q91V45 免疫原 KLH偶联的小鼠GPR54合成肽 抗体亚型 IgG 纯化方式 亲和纯化 亚细胞定位 细胞膜 应用 应用 物种 稀释 阳性样品 IHC/IF 免疫组织化学/免疫荧光 小鼠 1: 1500-1: 3000 心 背景 The protein encoded by this gene is a galanin-like G protein-coupled receptor that binds metastin, a peptide encoded by the metastasis suppressor gene KISS1. The tissue distribution of the expressed gene suggests that it is involved in the regulation of endocrine function, and this is supported by the finding that this gene appears to play a role in the onset of puberty. Mutations in this gene have been associated with hypogonadotropic hypogonadism and central precocious puberty. 图像 IHC检测GPR54蛋白(货号 GB112169). 样品: 小鼠心, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 3000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.