产品信息 蛋白质全称 尿嘧啶核苷酸/半胱氨酰白三烯受体 别名 UDP/CysLT receptor, G-protein coupled receptor 17, Gpr17, R12, Probable P2Y purinoceptor GPR17 Uniprot ID Q6NS65 免疫原 KLH偶联的小鼠GPCR GPR17合成肽 抗体亚型 IgG 预测分子量./观测分子量. 38 kDa / 50 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 人, 小鼠 1: 300-1: 800 SH-SY5Y细胞, 293T细胞, N2A细胞 背景 G protein-coupled receptor 17, GPR17, also known as uracil nucleotide/cysteinyl leukotriene receptor or P2Y-like receptor (P2YL), is a 367 amino acid member of the G-protein coupled receptor 1 family of proteins. While GPR17 is expressed in kidney, heart and umbilical vein endothelial cells, it is expressed in the highest levels in the brain. Upon brain injury, the extracellular concentrations of nucleotides and cysteinyl leukotrienes (CysLTs), two families of endogenous signaling molecules, increase significantly at the site of damage. 图像 WB检测GPCR GPR17蛋白(货号 GB112494). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 300稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.