产品信息 蛋白质全称 DNA-（无嘌呤/无嘧啶位点）裂解酶 别名 APEX nuclease, AP endonuclease 1, APEN, REF-1, Redox factor-1, Apex1, Ape, Ref1, Apex, APE1 Uniprot ID P28352 免疫原 KLH偶联的小鼠APE1合成肽 抗体亚型 IgG 预测分子量./观测分子量. 35 kDa / 35 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 人, 小鼠, 大鼠 1: 500-1: 1000 胰腺, 卵巢, 睾丸 背景 APEX1, also named as APE, APE1, HAP1 and REF-1, belongs to the DNA repair enzymes AP/ExoA family. It is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities are in DNA repair and redox regulation of transcriptional factors. In addition, APEX1 is also involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). 图像 WB检测APE1蛋白(货号 GB111199). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.