产品信息 蛋白质全称 泛素结合酶E2N 别名 Ube2n, Bendless like ubiquitin conjugating enzyme, BLU, UBC 13, Ube2n, Ubiquitin carrier protein N, Ubiquitin conjugating enzyme E2N Uniprot ID P61089 免疫原 小鼠UBE2N重组蛋白 抗体亚型 IgG2b, κ 克隆号 2C6E4 预测分子量./观测分子量. 17 kDa / 17 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 人, 小鼠, 大鼠 1: 1000-1: 2000 脑, 脊髓, 胸腺 背景 UBC13 is a member of the E2 ubiquitin-conjugating enzyme family. Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. Existing as a heterodimer with UBE2V2, UBC13 catalyzes the ATP-dependent synthesis of non-canonical polyubiquitin chains, a process that does not lead to proteasomal degradation. Additionally, UBC13 mediates the transcription of several target genes and is thought to play a role in cell cycle progression, cellular differentiation and DNA repair mechanisms that ensure cell survival after DNA damage. 图像 WB检测Ube2N/Ubc13蛋白(货号 GB12986). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗小鼠IgG (H+L) (货号GB23301), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.