产品信息 蛋白质全称 Flap 核酸内切酶1 别名 FEN-1, Flap structure-specific endonuclease 1, Fen1, Fen-1, DNase IV, flap structure endonuclease 1, hFEN 1, Maturation factor 1, MF1, RAD2 Uniprot ID P39749 免疫原 小鼠FEN1重组蛋白 抗体亚型 IgG 预测分子量./观测分子量. 42 kDa / 45 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 人, 小鼠 1: 500-1: 1000 肺, 睾丸 背景 FEN1(Flap endonuclease-1) is the prototypical member of the 5’-nuclease superfamily, whose activities span a range of cellular pathways involved in DNA replication and genome maintenance . FEN1 is a structure-selective metallonuclease essential for Okazaki fragment maturation through efficient removal of 5’ flaps resulting from strand displacement during lagging-strand synthesis. FEN1 is overexpressed in multiple cancer types, and has been suggested both as a biomarker relating to prognosis and disease progression and as a potential therapeutic target. 图像 WB检测FEN1蛋白(货号 GB111455). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.