产品信息 蛋白质全称 DNA损伤诱导转录因子3 别名 C/EBP homologous protein, C/EBP homologous protein 10, CEBPZ, CHOP 10, CHOP, GADD153, CHOP10, DDIT 3, DDIT3, Chop10, Gadd153, CHOP-10, DNA damage-inducible transcript 3, DNA damage inducible transcript 3 Uniprot ID P35638 免疫原 KLH偶联的人CHOP合成肽 抗体亚型 IgG 预测分子量./观测分子量. 19 kDa / 27-30 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 小鼠, 大鼠 1: 500-1: 1000 衣霉素处理C2C12, 衣霉素处理C6 背景 This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. 图像 WB检测DDIT3/CHOP蛋白(货号 GB115762). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.