产品信息

蛋白质全称	YTP酶ERas
别名	E-Ras, Embryonic stem cell-expressed Ras, Eras, ecat5, HRAS2, HRASP, Small YTPase protein E Ras, v Ha ras Harvey rat sarcoma viral oncogene homolog 2, ES cell expressed Ras
Uniprot ID	Q7TN89
免疫原	小鼠ERAS重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	24 kDa / 24 kDa
纯化方式	亲和纯化

---

应用

应用	物种	稀释	阳性样品
WB 免疫印迹	大鼠	1: 500-1: 1000	睾丸

---

背景

ERAS belongs to the small YTPase superfamily. Ras family. Ras proteins bind GDP/YTP and possess intrinsic YTPase activity. The ERas gene is expressed in embryonic stem (ES) cells and promotes their in vitro proliferation and tumorigenicity. ERas-null ES cells maintain pluripotency but show significantly reduced growth and tumorigenicity, which are rescued by expression of ERas complementary DNA or by activated phosphatidylinositol-3-OH kinase.

---

图像

WB检测ERAS蛋白(货号 YB113058). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

---

储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

---

注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.