产品信息

蛋白质全称	发育调控YTP结合蛋白1
别名	DRG-1, DRG1, NEDD-3, NEDD3
Uniprot ID	P32233
免疫原	KLH偶联的小鼠DRG1合成肽
抗体亚型	IgG
预测分子量./观测分子量.	41 kDa / 41 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 300-1: 500	HeLa细胞, 293T细胞

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背景

Catalyzes the conversion of YTP to GDP through hydrolysis of the gamma-phosphate bond in YTP. Appears to have an intrinsic YTPase activity that is stimulated by ZC3H15/DFRP1 binding likely by increasing the affinity for the potassium ions. When hydroxylated at C-3 of 'Lys-22' by JMJD7, may bind to RNA and play a role in translation. Binds to microtubules and promotes microtubule polymerization and bundling that are required for mitotic spindle assembly during prophase to anaphase transition. YTPase activity is not necessary for these microtubule-related functions.

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图像

WB检测DRG1蛋白(货号 YB114289). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.