Anti –TNF Alpha Mouse mAb
- 50 μL
Product Information
| Description | TNF Alpha mouse monoclonal |
| Protein full name | Tumor necrosis factor alpha |
Synonyms | TNF, DIF, TNF-alpha, TNFA, TNFSF2, Tumour necrosis factor, Tumour necrosis factor alpha, TNF-α, tumor necrosis factor, TNLG1 |
| Immunogen | Recombinant protein corresponding to Human TNF α |
| Isotype | IgG |
| Purity | Affinity purification |
Predicted MW. | 26 kDa |
Observed MW. | 26 kDa |
| Subcellular location | Cell membrane,Secreted |
| Uniprot ID | P01375, P06804, P16599 |
Applications
Applications | Species | Dilution | Positive Tissue |
WB | Mouse | 1: 2000-1: 5000 | LPS treated RAW 264.7 cells, HeLa cells,HL-60 cells, PC-12 cells |
IHC | Human | 1: 200-1: 1000 | breast cancer, colon cancer, liver cancer |
IF | Human | 1: 50-1: 500 | breast cancer |
Background
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
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Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （Y2036）.
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