产品信息

蛋白质全称	IL-3受体α亚基
别名	CD123, hIL 3Ra, IL-3 receptor subunit alpha, IL 3R alpha, IL-3R subunit alpha, IL-3RA, IL3R, IL3RA, IL-3RA, IL3RAY, IL3RX, IL3RY
Uniprot ID	P26952
免疫原	KLH偶联的小鼠IL3RA合成肽
抗体亚型	IgG
预测分子量./观测分子量.	43 kDa / 72 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 300-1: 1000	8226, THP-1细胞, U-87MG细胞

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背景

The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3.

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图像

WB检测IL3RA/CD123蛋白(货号 YB113833). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.