产品信息

蛋白质全称	肿瘤坏死因子配体超家族成员8
别名	CD153, CD30-L, CD30 ligand, CD30L, CD30LG, TNFSF8
Uniprot ID	P32972
免疫原	小鼠CD30-L重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	27 kDa / 27 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 300-1: 500	MCF-7细胞

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背景

TNFSF8, also named as CD30LG and CD153, is a member of the tumor necrosis factor (TNF) receptor superfamily, is a surface antigen used as a clinical marker for Hodgkin lymphoma and related hematologic malignancies. It induces proliferation of T-cells. CD30L enhanced the proliferation of CD3-activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines. For Glycosylation, the MW in WB detection is about 35-40 kDa.

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图像

WB检测CD30-L蛋白(货号 YB113400). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.