产品信息

蛋白质全称	脑蛋白样蛋白
别名	ABP1, Cervical SH3P7, CMAP, DBNL, Drebrin F, drebrin like, Drebrin like protein, HIP 55, SH3P7
Uniprot ID	Q62418
免疫原	KLH偶联的小鼠HIP55合成肽
抗体亚型	IgG
预测分子量./观测分子量.	49 kDa / 55 kDa
纯化方式	亲和纯化
亚细胞定位	细胞连接, 细胞膜, 细胞突起, 细胞质, 胞质囊泡

---

应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 1500-1: 2000	脑
ICC/IF 免疫细胞化学/免疫荧光	小鼠	1: 100-1: 400	NIH 3T3细胞

---

背景

Adapter protein that binds F-actin and DNM1, and thereby plays a role in receptor-mediated endocytosis. Plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. Does not bind G-actin and promote actin polymerization by itself. Required for the formation of organized podosome rosettes. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes.

---

图像

	WB检测HIP55蛋白(货号 YB114902). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.
	ICC检测HIP55蛋白(货号 YB114902)(红色). 样品: NIH-3T3细胞, 4%多聚甲醛 (货号Y1112) 固定20分钟. 封闭: 3% BSA（货号YC305021）的PBS溶液, 室温孵育30分钟. 一抗: 1: 200稀释, 4℃ 孵育过夜. 二抗: Cy3标记山羊抗兔IgG (H+L) (货号YB21314), 1: 300稀释, 室温孵育1小时.

---

储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

---

注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.