产品信息

蛋白质全称	Tropomodulin-4
别名	Skeletal muscle tropomodulin, Sk-Tmod, Tmod4
Uniprot ID	Q9JLH8
免疫原	小鼠TMOD4重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	39 kDa / 39 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 1000-1: 2000	心, 骨骼肌

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背景

TMOD4 is a member of tropomodulin (Tmod) family proteins which are capping proteins binding to the pointed end of actin filaments and preventing both elongation and depolymerization. Four mammalian Tmod genes have been identified to date. Tomd1 is expressed in terminally-differentiated post-mitotic cells, like heart, brain, lens, vasculature, and erythrocytes; Tmod2 expressed in neurons; Tmod3 expressed ubiquitously; Tmod4 expressed only in skeletal muscles. This antibody was raised against the full-length human TMOD4 and may cross-react with other Tmods.

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图像

WB检测TMOD4蛋白(货号 YB113198). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 2000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.