产品信息

蛋白质全称	Nesh-SH3靶标蛋白
别名	ABI3BP, Nesh binding protein, NESHBP, Target of Nesh SH3, TARSH
Uniprot ID	Q7Z7G0
免疫原	KLH偶联的人Tarsh合成肽
抗体亚型	IgG
预测分子量./观测分子量.	118 kDa / 72 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠	1: 800-1: 1200	胸腺

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背景

ABI3BP (ABI gene family member 3-binding protein) contains 2 fibronectin type-III domains and is thought to interact with ABI3. It is expressed in brain, heart, lung, liver, pancreas, kidney and placenta. ABI3BP expression is dramatically reduced in human lung cancer cell lines and primary lung tumor, while it is predominantly expressed in normal conditions, suggesting that it is involved in prevention of tumorigenesis.

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图像

WB检测Tarsh蛋白(货号 YB114923). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 800稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.