产品信息

蛋白质全称	纤维调节蛋白
别名	FM, FMOD, KSPG fibromodulin, SLRR2E, Collagen binding 59 kDa protein, Keratan sulfate proteoglycan fibromodulin, Fibromodulin precursor
Uniprot ID	P50608
免疫原	小鼠Fibromodulin重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	43 kDa / 45 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1000	HepG2细胞, A431细胞, Caco2细胞

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背景

Fibromodulin (FMOD), an extracellular matrix protein, belongs to the small leucine-rich proteoglycan family. Fibromodulin is a collagen-binding protein present in many types of connective tissues, e.g. cartilage, tendon, skin, sclera and cornea . It is involved in fibrillogenesis, cell adhesion, and cytokine activity modulation . Fibromodulin may participate in the assembly of the extracellular matrix as it interacts with type I and type II collagen fibrils and inhibits fibrillogenesis in vitro.

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图像

WB检测Fibromodulin蛋白(货号 YB113526). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.