产品信息

蛋白质全称	Ena/VASP样蛋白
别名	Ena/vasodilator-stimulated phosphoprotein-like, Enah/Vasp-like RNB6Ena/vasodilator-stimulated phosphoprotein-like
Uniprot ID	P70429
免疫原	小鼠EVL重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	45 kDa / 52 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 500-1: 1000	胸腺, 脑, 淋巴结

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背景

Enah/Vasp-like (EVL) is a member of the Ena/VASP protein family. Ena/VASP proteins are actin-associated proteins involved in a range of processes that require dynamic actin remodeling such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization. There are two different isoforms, generated by alternative splicing.

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图像

WB检测EVL蛋白(货号 YB111037). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.