产品信息

蛋白质全称	双链RNA结合蛋白Staufen同源物1
别名	Stau1, Stau, staufen, Staufen RNA binding protein homolog 1, PPP1R150, FLJ25010, Staufen/STAU1
Uniprot ID	Q9Z108
免疫原	小鼠Staufen/STAU1重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	54 kDa / 54 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠	1: 500-1: 1000	HepG2细胞, A431细胞, K562细胞

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背景

Staufen1 (STAU1)-mediated mRNA decay (SMD) degrades translationally active mRNAs that bind the double-stranded (ds)RNA binding protein STAU1 within their 3'-untranslated regions (3'UTRs) [PMID:21307942]. STAU1 can binds to stem structures and protect Dvl2 mRNA from degradation by associating with the secondary structure in the U1 region which is located at 5′ position of second U stretch in the U2 region [PMID:17510634]. It has a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site.

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图像

WB检测Staufen/STAU1蛋白(货号 YB111935). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.