产品信息

蛋白质全称	α1-抗胰蛋白酶
别名	AAT, Alpha-1 protease inhibitor 2, Alpha-1-antiproteinase, Serine protease inhibitor 1-2, Serine protease inhibitor A1b, Serpin A1b, Serpina1b, Aat2, Dom2, Spi1-2
Uniprot ID	P01009
免疫原	KLH偶联的人alpha 1 Antitrypsin合成肽
抗体亚型	IgG
预测分子量./观测分子量.	47 kDa / 51-55 kDa
纯化方式	亲和纯化

---

应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 300-1: 400	MCF7细胞

---

背景

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.; [Short peptide from AAT]: Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

---

图像

WB检测alpha 1 Antitrypsin蛋白(货号 YB115567). 样品: 经RIPA裂解液(货号Y2013)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号YC310012)溶于TBST溶液, 室温孵育1小时. —抗: 1: 300稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号YB23314), 1: 5000稀释, 室温孵育1小时.

---

储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

---

注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(Y2036)稀释本产品.