Gene Name Synonym

PDGF, subunit B, PDGF-BB, PDGF-2, Pdgfb, PDGFBB

Species

Rat

Accession

Q05028

Source

E.coli

SPECIFICATIONS

Amino acid sequence:

E.coli-derived Rat PDGFB protein, Ser 74-Thr 182.

Molecular Weight:

Approximately 12.2 kDa.

Purity:

>97.0% as determined by SDS-PAGE.

Endotoxin:

<1.0 EU/μg of the protein by the LAL method.

Formulation:

Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution:

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Platelet-derived growth factor-B (PDGFB) protein is a key growth factor that centrally regulates embryonic development, cell proliferation, migration, survival, and chemotaxis. It is known for its potent mitogenic effects on mesenchymal cells and is essential for the normal proliferation and recruitment of pericytes and vascular smooth muscle cells in tissues such as the central nervous system, skin, lungs, heart, and placenta. PDGFB is necessary for normal cardiovascular development. The administration of PDGFB alone normalized tumor vasculature by increasing periendothelial coverage and vascular functionality. Interestingly, this effect exerted by PDGFB was also observed in the presence of DAPT. So PDGFB is able to improve tumor vascularity and allows the anticancer action of DAPT in the tumor.

Data

SDS-PAGE

Bioactivity

Recombinant Rat PDGFB Protein was determined by SDS-PAGE with Coomassie Blue.

Measured in a cell proliferation assay using Balb/c 3T3 A31 mouse embryonic fibroblasts.  The ED50 for this effect is typically 0.01-0.05 μg/mL.