Gene Name Synonym

FGF-1, FGF1, HBGF-1, FGFA, aFGF

Species

Mouse

Accession

P61148

Source

E.coli

SPECIFICATIONS

Amino acid sequence

E.coli-derived Mouse aFGF protein, Phe16-Asp155.

Molecular Weight

Approximately 16.0 kDa.

Purity

>97.0% as determined by SDS-PAGE.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Formulation

Lyophilized from sterile Tris-Hcl, pH 8.0. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Data

SDS-PAGE

Bioactivity

Recombinant Mouse aFGF Protein was determined by SDS-PAGE with Coomassie Blue.

Measured in a cell proliferation assay using Balb/C 3T3 A31 mouse embryonic fibroblast cells. The ED50 for this effect is 0.01-0.05 μg/mL.