Gene Name Synonym

LR3 IGF-I, IBP1, IGF1, IGF-1, IGF1A, IGFI, IGF-I, IGF-IA, IGF-IB, MGF

Species

Human

Accession

P05019

Source

E.coli

SPECIFICATIONS

Amino acid sequence

E.coli-derived Human LR3 IGF-1 protein with additional 13 aa at N terminal, Gly49-Ala118.

Molecular Weight

Approximately 9.0 kDa.

Purity

>95.0% as determined by SDS-PAGE.

Endotoxin

<1.0 EU/μg of the protein by the LAL method.

Formulation

Lyophilized from sterile Tris-Hcl, pH 8.0. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Long R3 IGF-I (LR3 IGF-I) is a 9.2 kDa synthetic analog of IGF-I that is generated by modifying the aa sequence for mature human IGF-I. The LR3IGF-1 is a long-term analog of human IGF-1, specifically designed and manufactured for mammalian cell culture to support large-scale manufacturing of recombinant biopharmaceuticals. IGF-1 belonged to the insulin gene family, is a mitogenic polypeptide growth factor that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. The body growth of animals is regulated by growth hormone and IGF-I. The classical theory of this regulation is that most IGF-I in the blood originates in the liver and that body growth is controlled by the concentration of IGF-I in the blood. Meanwhile, IGF-I has been shown to enhance neuronal survival and inhibit apoptosis.

Data

SDS-PAGE

Bioactivity

Recombinant Human LR3 IGF-1 Protein was determined by SDS-PAGE with Coomassie Blue.

Measured in a cell proliferation assay using PDC-P1 cells. The ED50 for this effect is 0.1-0.5 μg/mL.