Gene Name Synonym

C7, IFI10, INP10, IP-10, crg-2, mob-1, SCYB10, gIP-10, CXCL10

Species

Mouse

Accession

P17515

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Ile22-Pro98 is expressed with a 10×His tag at the C terminus

Molecular Weight

8.7 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

C-X-C Motif Chemokine Ligand 10 (CXCL10) is a pro-inflammatory chemokine secreted by a wide spectrum of cells. It is involved in multiple mechanisms and reported to have pleiotropic effects, including immune cell migration and attraction to inflammation sites, angiogenesis, and cancer cell growth. CXCL10 is produced by pancreatic islet cells upon inflammatory stress and is specifically recognized by C-X-C Motif Chemokine Receptor 3 (CXCR3) which is expressed by activated T-lymphocytes and other immune cells.

Data

SDS-PAGE

Bioactivity

Recombinant Mouse CXCL10 Protein was determined by SDS-PAGE with Coomassie Blue.

Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC-T1). The ED50 for this effect is typically 0.05-0.1 μg/mL.