Gene Name Synonym

IL-9, Interleukin-9, IL9, Cytokine P40

Species

Mouse

Accession

P15247

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Gln19-Pro144 is expressed with a 10×His tag at the C terminus

Molecular Weight

15.6 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Interleukin 9, also known as IL-9, is a cytokine (cell signaling molecule) belonging to the group of interleukins. IL-9 is a multifunctional cytokine secreted by TH2 lymphocytes. Interleukin-9 is reported to play important roles in innate and adaptive immunity in extrarenal inflammatory diseases. The tolerogenic cytokine IL9 promotes T regulatory cell function and allergic airway inflammation. Eliminating endogenous IL9 enabled sensitization of host T cells to tumors, leading to their early rejection without the requirement of vaccines or immunomodulatory therapies. IL-9 predominantly produced by TH17 cells synergizes with TGF-β1 to differentiate naive CD4+ T cells into TH17 cells, while IL-9 secretion by TH17 cells is regulated by IL-23. IL-9 has been demonstrated to be involved in several diseases, such as cancer, autoimmunity and other pathogen-mediated immune-regulated diseases.

Data

SDS-PAGE

Bioactivity

Recombinant Mouse IL-9 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at30-40 kDa.

Measured in a cell proliferation assay using YC/9 mouse mast cells. The ED50 for this effect is 1-5 ng/mL.