Gene Name Synonym

IL-19, IL19, Interleukin-19, ZMDA1, NG.1

Species

Human

Accession

Q9UHD0

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Leu25-Ala177 is expressed with a 10×His tag at the C terminus

Molecular Weight

19.0 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU·mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Interleukin- (IL-) 19, part of the IL-10 family, contributes to a range of diseases and disorders, such as asthma, endotoxic shock, uremia, psoriasis, and rheumatoid arthritis. IL-19 is expressed in several types of tumor cells, especially in squamous cell carcinoma of the skin, tongue, esophagus, and lung and invasive duct carcinoma of the breast.  Interleukin (IL) 19 have been identified to play a role in the regulation of epidermal functions and in inflammation. IL-19 is a type of anti-inflammatory cytokine. IL19 expression was stimulated by diverse cellular stresses, including inhibition of the DNA replication checkpoint kinase ATR (ataxia telangiectasia and Rad3-related protein), oncogene expression, replicative exhaustion, oxidative stress, and DNA double-strand breaks. The molecular features at the IL19 locus may modestly alter the establishment of HIV-1 infection.

Data

SDS-PAGE

Bioactivity

Active Recombinant Human IL-19 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 22-30 kDa.

Determined by its ability to induce STAT3 activation in Hela STAT3 LUC reporter cells.The ED50 for this effect is 5-10 ng/mL.