Gene Name Synonym

IL-6, IL6, Interleukin-6, BSF2, HSF, IFNB2

Species

Mouse

Accession

P08505

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Phe25-Thr211 is expressed with a 10×His tag at the N terminus

Molecular Weight

23.2 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU·mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL6 is a potent inducer of the acute phase response. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as a phosphorylated and variably glycosylated molecule.IL-6 can lead to block the PI3K/AKT pathway. It exerts actions through its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6.

Data

SDS-PAGE

Bioactivity

Active Recombinant Mouse IL-6 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 20-30 kDa.

Measured in a cell proliferation assay using M-NFS-60 mouse B cell. The ED50 for this effects is 0.1-1 ng/mL.