Gene Name Synonym

IL-5, IL5, TRF, IL5, Interleukin-5

Species

Mouse

Accession

P04401

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Met21-Gly133 is expressed with a 10×His tag at the C terminus

Molecular Weight

14.4 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU·mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

IL-5 critically regulates expression of genes involved in cell survival, IgH switch recombination, maturation in B cells and genes required for growth, survival, and effector function of eosinophils. IL-5 was recognized as the major maturation and differentiation factor for eosinophils in mice and humans. IL-5 was originally defined as a T-cell-derived cytokine that triggers activated B cells for terminal differentiation into antibody-secreting plasma cells. IL-5 critically regulates expression of genes involved in proliferation, cell survival and maturation and effector functions of B cells and eosinophils. Growth in the number of circulating, airway tissue, and induced sputum eosinophils have been observed in patients with these diseases. Interleukin 5 has long been associated with several allergic diseases, including allergic rhinitis and asthma.

Data

SDS-PAGE

Bioactivity

Active Recombinant Mouse IL-5 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 22-30 kDa.

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 70 ng/mL.