Gene Name Synonym

IL-13, IL13, NC30, ALRH, BHR1, MGC116786, MGC116788, MGC116789, P600, Interleukin-13

Species

Mouse

Accession

P20109

Source

CHO Cells

SPECIFICATIONS

Amino acid sequence

Ala19-Phe131 is expressed with a 10×His tag at the C terminus

Molecular Weight

13.8 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU·mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Interleukin 13 (IL-13) is a single-chain glycosylated polypeptide, which belongs to the IL-13/IL-4 family. Some time ago, the Th2 cytokine, interleukin (IL)-13, was identified as a critical regulator of the allergic response. IL-13, a T-helper type 2 (Th2) cytokine, was first described in 1993, identified by molecular cloning in activated human T lymphocytes. IL-13 was reported to direct cells towards the Th2 pathway, with induction of B cell production of IgE. As a cytokine, IL-13 protein is critical in regulating inflammatory, immune responses, and diseases. Also, it inhibits the production of pro-inflammatory cytokines and chemokines, and thus down-regulates macrophage activity. In particular, IL-13 up-regulation is associated with severe disease.

Data

SDS-PAGE

Bioactivity

Active Recombinant Mouse IL-13 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 18-30 kDa.

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 1-10 ng/mL.