Gene Name Synonym

Oncostatin M,oncostatin-M,OSM

Species

Human

Accession

P13725

Source

E.coli

SPECIFICATIONS

Amino acid sequence:

A DNA sequence encoding the Human OSM protein(Ala 26-Arg 221) was expressed with a 6×His tag at the N terminus.

Molecular Weight:

Approximately 23.1 kDa.

Purity:

>95.0% as determined by SDS-PAGE

Endotoxin:

<1.0 EU/μg of the protein by the LAL method.

Formulation:

Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution:

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Oncostatin M (OSM) is a multifunctional growth regulator belonging to the interleukin-6 subfamily of cytokines that has dual functions of inhibiting tumor cell lines and stimulating the proliferation of AIDS-KS cells. OSM is considered as a pleiotropic cytokine that signals through cell surface receptors typeⅠand typeⅡ both of which share the similarity of containing protein gp130 and takes part in many bio metabolism processes including liver development, hematopoiesis, inflammation, bone formation, and destruction and possibly CNS development. OSM was previously identified by its ability to inhibit the growth of cells from melanoma and other solid tumors. It also has been reported that OSM, like LIF, IL-6, and G-CSF, can inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells. OSM is synthesized by stimulated T-cells and monocytes. Furthermore, the effects of OSM on endothelial cells suggest a pro-inflammatory role for OSM and endothelial cells possess a large number of OSM receptors.

Data

SDS-PAGE

Bioactivity

Recombinant Human OSM Protein was determined by SDS-PAGE with Coomassie Blue.