Anti-alpha Actinin/ACTN1 Rabbit pAb
- 100 μL
Product Information
Description | alpha Actinin/ACTN1 rabbit polyclonal |
Protein full name | Alpha-actinin-1 |
Synonyms | Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1, ACTN1, |
Immunogen | KLH conjugated Synthetic peptide corresponding to Human alpha Actinin/ACTN1 |
Isotype | IgG |
Purity | Affinity purification |
Subcellular location | |
Predicted MW. | 103 kDa |
Observed MW. | 103 kDa |
Uniprot ID |
Applications
Applications | Species | Dilution | Positive Tissue |
WB | Human, Mouse, Rat | 1: 500-1: 800 | kidney, muscle, uterus |
IHC | Human, Mouse, Rat | 1: 300-1: 600 | brain, skin, uterus, heart |
IF | Human, Mouse, Rat | 1: 300-1: 600 | skin, skeletal muscle, uterus |
Background
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane.This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene.
Images
| Western blot analysis of ACTN1 (YB112139) at dilution of 1: 800 Lane 1: HeLa cell lysate Lane 2: NIH3T3 cell lysate Lane 3: Mouse kidney tissue lysate Lane 4: Mouse muscle tissue lysate Lane 5: Rat uterus tissue lysate |
| Immunohistochemistry analysis of paraffin-embedded human brain using ACTN1 (YB112139) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin-embedded human skin using ACTN1 (YB112139) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin-embedded mouse uterus using ACTN1 (YB112139) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin-embedded rat uterus using ACTN1 (YB112139) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin-embedded rat heart using ACTN1 (YB112139) at dilution of 1: 600 |
| Immunofluorescent analysis of paraformaldehyde-fixed human skin using ACTN1 (YB112139) at dilution of 1: 600 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse skeletal muscle using ACTN1 (YB112139) at dilution of 1: 600 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat skeletal muscle using ACTN1 (YB112139) at dilution of 1: 600 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse uterus using ACTN1 (YB112139) at dilution of 1: 600 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat uterus using ACTN1 (YB112139) at dilution of 1: 600 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （Y2036）.
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